Anti-BRAF Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

BA2958-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of BRAF using anti-BRAF antibody (BA2958-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Brain tissue lysates,
Lane 2: Rat Testis tissue lysates,
Lane 3: Rat Liver tissue lysates,
Lane 4: SW620 whole cell lysates,
Lane 5: COLO320 whole cell lysates,
Lane 6: PC-12 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BRAF antigen affinity purified polyclonal antibody (BA2958-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BRAF at approximately 85 kDa. The expected band size for BRAF is at 84 kDa.
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产品简介>

产品名称

Anti-BRAF Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

B RAF1; BRAF; BRAF1; p94; Proto oncogene B Raf; RAFB1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

BRAF

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human B Raf, identical to the related rat and mouse sequences.

计算分子量

84 kDa

实际分子量

85 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

BRAF(v-raf murine sarcoma viral oncogene homolog B1) is a human gene that makes a protein called B-Raf, also known as ONCOGENE BRAF, BRAF1, RAFB1, Proto-oncogene B-Raf p94, v-Raf murine sarcoma viral oncogene homolog B1, proto-oncogene B-Raf, v-Raf murine sarcoma viral oncogene homolog B1, while the protein is more formally known as serine/threonine-protein kinase B-Raf. BRAF deduced 651-amino acid protein has a calculated molecular mass of 72.5 kD. Northern blot analysis detected transcripts of 10 and 13 kb in cerebrum, fetal brain, and placenta and transcripts of 2.6 and 4.5 kb in testis. Testis also showed lower expression of the 10- and 13-kb transcripts.B-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion.

Uniprot ID

P15056

文献引用格式

BRAF Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA2958-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

v-raf murine sarcoma viral oncogene homolog B1

蛋白名全称

Serine/threonine-protein kinase B-raf

实验方案>

实验步骤

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