Anti-ALDH2 Antibody (Clone#6H2)

筛选器： All WB ICC/IF FCM

• 

  Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human HEK293 whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human SHG-44 whole cell lysates,
  Lane 6: human THP-1 whole cell lysates,
  Lane 7: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (M00546-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.

• 

  Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat kidney tissue lysates,
  Lane 3: rat heart tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse liver tissue lysates,
  Lane 6: mouse Ana-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (M00546-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.

• 

  IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2).
  ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2).
  ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Flow Cytometry analysis of SiHa cells using anti-ALDH2 antibody (M00546-2).
  Overlay histogram showing SiHa cells stained with M00546-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ALDH2 Antibody (M00546-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human HepG2 whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human HEK293 whole cell lysates,
  Lane 4: human HL-60 whole cell lysates,
  Lane 5: human SHG-44 whole cell lysates,
  Lane 6: human THP-1 whole cell lysates,
  Lane 7: human K562 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (M00546-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.

• 

  Western blot analysis of ALDH2 using anti-ALDH2 antibody (M00546-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: rat liver tissue lysates,
  Lane 2: rat kidney tissue lysates,
  Lane 3: rat heart tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse liver tissue lysates,
  Lane 6: mouse Ana-1 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-ALDH2 antigen affinity purified monoclonal antibody (M00546-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.

• 

  IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2).
  ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  IF analysis of ALDH2 using anti-ALDH2 antibody (M00546-2).
  ALDH2 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 2μg/mL mouse anti-ALDH2 Antibody (M00546-2) overnight at 4°C. DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Flow Cytometry analysis of SiHa cells using anti-ALDH2 antibody (M00546-2).
  Overlay histogram showing SiHa cells stained with M00546-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ALDH2 Antibody (M00546-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.