Anti-ALDH2 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC ICC/IF

PB0494

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of ALDH2 using anti-ALDH2 antibody (PB0494). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Kidney tissue lysates,
Lane 2: Mouse Kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-ALDH2 antigen affinity purified polyclonal antibody (PB0494) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for ALDH2 at approximately 56 kDa. The expected band size for ALDH2 is at 56 kDa.
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IHC analysis of ALDH2 using anti-ALDH2 antibody (PB0494).
ALDH2 was detected in a paraffin-embedded section of rat liver tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALDH2 Antibody (PB0494) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of ALDH2 using anti-ALDH2 antibody (PB0494).
ALDH2 was detected in a paraffin-embedded section of human renal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-ALDH2 Antibody (PB0494) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IF analysis of ALDH2 using anti-ALDH2 antibody (PB0494).
ALDH2 was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-ALDH2 Antibody (PB0494) at a dilution of 1:100. Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1135) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(4)

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产品简介>

产品名称

Anti-ALDH2 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

ALDH class 2; ALDH E2; ALDH2; ALDHI; ALDM

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, ICC/IF

基因名称

ALDH2

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human ALDH2 different from the related mouse sequence by two amino acids, and from the related rat sequence by one amino acid.

计算分子量

56 kDa

实际分子量

56 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

ALDH2 (Aldehyde Dehydrogenase 2 Family) is a human gene. The enzyme encoded by this gene belongs to the aldehyde dehydrogenase family of enzymes that catalyze the chemical transformation from acetaldehyde to acetic acid. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Hsu et al. (1985) assigned the ALDH2 locus to chromosome 12 by means of a cDNA probe and Southern blot analysis of somatic cell hybrids. Using an unbiased proteomic search, Chen et al. (2008) identified mitochondrial ALDH2 as an enzyme whose activation correlated with reduced ischemic heart damage in rodent models. A high-throughput screen identified a small molecule activator of ALDH2, which they called Alda-1, that, when administered to rats before an ischemic event, reduced infarct size by 60%, most likely through its inhibitory effect on the formation of cytotoxic aldehydes.

Uniprot ID

P05091

文献引用格式

ALDH2 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0494)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

aldehyde dehydrogenase 2 family (mitochondrial)

蛋白名全称

Aldehyde dehydrogenase, mitochondrial

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