Anti-Calcium Sensing Receptor/CASR Antibody (Clone#11E9)

筛选器： All WB FCM

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  Western blot analysis of anti-Calcium Sensing Receptor/CASR antibody (M00574). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysate,
  Lane 2: human A549 whole cell lysate,
  Lane 3: human 22RV1 whole cell lysate,
  Lane 4: human HepG2 whole cell lysate,
  Lane 5: human Caco-2 whole cell lysate.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Calcium Sensing Receptor/CASR antigen affinity purified monoclonal antibody (M00574) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calcium Sensing Receptor/CASR at approximately 130 kDa. The expected band size for Calcium Sensing Receptor/CASR is at 121 kDa.

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  Flow Cytometry analysis of Neuro-2a cells using anti-Calcium Sensing Receptor/CASR antibody (M00574).
  Overlay histogram showing Neuro-2a cells stained with M00574 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calcium Sensing Receptor/CASR Antibody (M00574) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of anti-Calcium Sensing Receptor/CASR antibody (M00574). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysate,
  Lane 2: human A549 whole cell lysate,
  Lane 3: human 22RV1 whole cell lysate,
  Lane 4: human HepG2 whole cell lysate,
  Lane 5: human Caco-2 whole cell lysate.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Calcium Sensing Receptor/CASR antigen affinity purified monoclonal antibody (M00574) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Calcium Sensing Receptor/CASR at approximately 130 kDa. The expected band size for Calcium Sensing Receptor/CASR is at 121 kDa.

• 

  Flow Cytometry analysis of Neuro-2a cells using anti-Calcium Sensing Receptor/CASR antibody (M00574).
  Overlay histogram showing Neuro-2a cells stained with M00574 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Calcium Sensing Receptor/CASR Antibody (M00574) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.