Anti-SSB Antibody (Clone#OTI1E11)

Mouse monoclonal antibody

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筛选器： All WB ICC/IF FCM

M00705-1

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSB monoclonal antibody.
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Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-SSB monoclonal antibody at 1:1000 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSB monoclonal antibody (1:200).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSB (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSB(Cat# M00705-1).
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Immunofluorescent staining of HepG2 cells using anti-SSB mouse monoclonal antibody.
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Anti-SSB mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSB .
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Flow cytometric Analysis of Jurkat cells, using anti-SSB antibody, (Red), compared to a nonspecific negative control antibody, (Blue).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-SSB Antibody (Clone#OTI1E11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

La; La autoantigen; La ribonucleoprotein; LARP3; Lupus La protein; SS B; SSB

产品类型

Monoclonal

检验物种

human, mouse, rat, dog, monkey

应用范围

WB, ICC/IF, FCM

基因名称

SSB

克隆号

OTI1E11

宿主

Mouse

抗体亚型

IgG2a

免疫原

Full-length protein expressed in 293T cell transfected with human SSB expression vector

实际分子量

47 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

Lot dependent

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

La is involved in diverse aspects of RNA metabolism, including binding and protecting 3-prime UUU(H) elements of newly RNA polymerase III (see MIM 606007)-transcribed RNA, processing 5-prime and 3-prime ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAsassociated with hepatitis C virus. La protein was originally defined by its reactivity with autoantibodies from patients with Sjogren syndrome (MIM 270150) and systemic lupus erythematosus

Uniprot ID

P05455

文献引用格式

SSB Antibody (Clone#OTI1E11) (Boster Biological Technology, Wuhan, China. Catalog#M00705-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

small RNA binding exonuclease protection factor La

蛋白名全称

Lupus La protein

实验方案>

实验步骤

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