Anti-Apolipoprotein AI/APOA1 Antibody (Clone#17G5)

Mouse monoclonal antibody

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筛选器： All WB ELISA(Cap)

M00717-1

100μg ￥2860
货期：现货

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Western blot analysis of Apolipoprotein AI/APOA1 using anti-Apolipoprotein AI/APOA1 antibody (M00717-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Apolipoprotein AI/APOA1 antigen affinity purified monoclonal antibody (M00717-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Apolipoprotein AI/APOA1 at approximately 25 kDa. The expected band size for Apolipoprotein AI/APOA1 is at 31 kDa.
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产品名称

Anti-Apolipoprotein AI/APOA1 Antibody (Clone#17G5)

规格/价格

100μg/2860

指标别称

Apo AI; ApoA I; APOA1; apolipoprotein A I; Apolipoprotein A1

产品类型

Monoclonal

检验物种

human

应用范围

WB, ELISA(Cap)

基因名称

APOA1

克隆号

17G5

宿主

Mouse

抗体亚型

IgG1

免疫原

E. coli-derived mouse Apolipoprotein A I recombinant protein (Position: D25-Q264). Mouse Apolipoprotein A I shares 64% and 68.6% amino acid (aa) sequence identity with human and rat Apolipoprotein A I, respectively.

计算分子量

31 kDa

实际分子量

25 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Apolipoprotein A-1, also known as APOA1, is a human protein with a specific role in lipid metabolism. It binds to lipopolysaccharide or endotoxin, and has a major role in the anti-endotoxin function of HDL. The gene is mapped to 11q23. And it is a single polypeptide chain with 243 amino acid residues of known primary amino acid sequence. The ApoA-I protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I is also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. Additionally, ApoA-I overexpression promotes macrophage-specific reverse cholesterol transport.

Uniprot ID

P02647

文献引用格式

Apolipoprotein AI/APOA1 Antibody (Clone#17G5) (Boster Biological Technology, Wuhan, China. Catalog#M00717-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

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