Anti-TGFBR2 Antibody (Clone#2F11)

mouse monoclonal antibody

说明书一键复制产品信息

筛选器： All WB IHC ICC/IF FCM

M00759-2

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

加入购物车立即购买

Western blot analysis of TGFBR2 using anti-TGFBR2 antibody (M00759-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HEPG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human K562 whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: human HELA whole cell lysates,
Lane 6: human T47D whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-TGFBR2 antigen affinity purified monoclonal antibody (M00759-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TGFBR2 at approximately 70-85 kDa. The expected band size for TGFBR2 is at 65 kDa.
all(6)
IHC analysis of TGFBR2 using anti-TGFBR2 antibody (M00759-2).
TGFBR2 was detected in a paraffin-embedded section of human placenta tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TGFBR2 Antibody (M00759-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of TGFBR2 using anti-TGFBR2 antibody (M00759-2).
TGFBR2 was detected in a paraffin-embedded section of human cervical intraepithelial neoplasia tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TGFBR2 Antibody (M00759-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IHC analysis of TGFBR2 using anti-TGFBR2 antibody (M00759-2).
TGFBR2 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-TGFBR2 Antibody (M00759-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.
all(6)
IF analysis of TGFBR2 using anti-TGFBR2 antibody (M00759-2).
TGFBR2 was detected in an immunocytochemical section of HepG2 cells. The section was incubated with mouse anti-TGFBR2 Antibody (M00759-2) at a dilution of 1:100. Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1126) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
all(6)
Flow Cytometry analysis of A549 cells using anti-TGFBR2 antibody (M00759-2).
Overlay histogram showing A549 cells stained with M00759-2 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-TGFBR2 Antibody (M00759-2) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
all(6)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-TGFBR2 Antibody (Clone#2F11)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AAT3; FAA3; LDS1B; LDS2B; MFS2; RIIC; TAAD2; TbetaR II; TGF beta receptor type 2; TGF beta receptor type II; TGF beta type II receptor; TGFbeta RII; TGFBR2; TGFR 2

产品类型

Monoclonal

检验物种

human

应用范围

WB, IHC, ICC/IF, FCM

基因名称

TGFBR2

克隆号

2F11

宿主

Mouse

抗体亚型

IgG2b

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human TGFBR2, different from the related mouse sequence by five amino acids, and from the related rat sequence by eight amino acids.

计算分子量

65 kDa

实际分子量

70-85 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

protein G purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TGFBR2 (transforming growth factor, beta receptor II (70/80kDa)), also known as TGF-beta receptor type-2, TGFR-2, TGF-beta type II receptor, Transforming growth factor-beta receptor type II(TGF-beta receptor type II, TbetaR-II), is a member of the Ser/Thr protein kinase family and the TGFB receptor subfamily. A TGFBR2 cDNA encodes a deduced 565-amino acid protein with a calculated molecular mass of approximately 60 kD in length. The encoded protein is a transmembrane protein that has a protein kinase domain, forms a heterodimeric complex with another receptor protein, and binds TGF-beta. This receptor/ligand complex phosphorylates proteins, which then enter the nucleus and regulate the transcription of a subset of genes related to cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. Alternatively spliced transcript variants encoding different informs have been characterized.

Uniprot ID

P37173

RRID

AB_3081797

文献引用格式

TGFBR2 Antibody (Clone#2F11) (Boster Biological Technology, Wuhan, China. Catalog#M00759-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transforming growth factor, beta receptor II (70/80kDa)

蛋白名全称

TGF-beta receptor type-2

实验方案>

实验步骤

引用文献(0)>

清除