Western blot (WB): | 1:2000 |
Flow Cytometry (FCM): | 1:100 |
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63.
Flow cytometric Analysis of MCF-7 cells, using anti-CD63 antibody, (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD63 antibody, and then analyzed by flow cytometry (1:100).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD63 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD63.
Flow cytometric Analysis of MCF-7 cells, using anti-CD63 antibody, (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100)
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-CD63 antibody, and then analyzed by flow cytometry (1:100).