Anti-CD63 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9250

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of CD63 using anti-CD63 antibody (PB9250). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MDA-MB-453 whole cell lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human HL-60 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD63 antigen affinity purified polyclonal antibody (PB9250) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD63 at approximately 30-60 kDa. The expected band size for CD63 is at 26 kDa.
all(3)
IHC analysis of CD63 using anti-CD63 antibody (PB9250) .
CD63 was detected in a paraffin-embedded section of human bladder urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-CD63 Antibody (PB9250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of CD63 using anti-CD63 antibody (PB9250) .
CD63 was detected in a paraffin-embedded section of human spleen tissue. The tissue section was incubated with rabbit anti-CD63 Antibody (PB9250) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品简介>

产品名称

Anti-CD63 Antibody

规格/价格

50μl|1280 100μl|2180 150μl|2800

指标别称

CD63; CD63 antigen; CD63 molecule; Cell and organelle markers; Endosome Marker; Exosome marker; Granulophysin; LAMP 3; ME491; MLA1; OMA81H; Tetraspanin 30; Tspan 30; TSPAN30

产品类型

Polyclonal

检验物种

human

应用范围

WB, IHC

基因名称

CD63

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human CD63 recombinant protein (Position: E97-M238). Human CD63 shares 74% and 73% amino acid (aa) sequence identity with mouse and rat CD63, respectively.

计算分子量

26 kDa

实际分子量

26/60 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

CD63 antigen is a protein that in humans is encoded by the CD63 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. It is mapped to 12q13.2. CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression.

Uniprot ID

P08962

RRID

AB_3082035

文献引用格式

CD63 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9250)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

CD63 molecule

蛋白名全称

CD63 antigen

实验方案>

实验步骤

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