Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-200 |
Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-200 |
ImmunoPrecipitation (IP): | 1:50 |
Flow Cytometry (FCM): | 1:50 |
Western blot analysis of AP2 alpha expression in Hela cell lysate.
Western blot analysis of anti-TFAP2A antibody (M01167). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAP2A antigen affinity purified monoclonal antibody (M01167) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAP2A at approximately 48 kDa. The expected band size for TFAP2A is at 48 kDa.
Western blot analysis of AP2 alpha expression in Hela cell lysate.
Western blot analysis of anti-TFAP2A antibody (M01167). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SIHA whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: rat C6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-TFAP2A antigen affinity purified monoclonal antibody (M01167) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for TFAP2A at approximately 48 kDa. The expected band size for TFAP2A is at 48 kDa.