Anti-AP-2/TFAP2A Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0096

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of AP-2/TFAP2A using anti-AP-2/TFAP2A antibody (PB0096).
Lane 1: recombinant human AP2A protein 0.5ng.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AP-2/TFAP2A antigen affinity purified polyclonal antibody (PB0096) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AP-2/TFAP2A at approximately 38 kDa.
all(2)
Western blot analysis of AP-2/TFAP2A using anti-AP-2/TFAP2A antibody (PB0096). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AP-2/TFAP2A antigen affinity purified polyclonal antibody (PB0096) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AP-2/TFAP2A at approximately 48 kDa. The expected band size for AP-2/TFAP2A is at 48 kDa.
all(2)

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产品名称

Anti-AP-2/TFAP2A Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Activator protein 2; AP 2; AP 2 transcription factor; AP 2alpha; AP2; AP2 alpha; AP2TF; BOFS; TFAP2; TFAP2A; TFAP2A; AP-2

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

TFAP2A

宿主

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human AP2 alpha recombinant protein (Position: M1-G166). Human AP2 alpha shares 98% amino acid (aa) sequence identity with both mouse and rat AP2 alpha.

计算分子量

48 kDa

实际分子量

48 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

TFAP2A, also known as AP-2alpha or BOFS, is a protein that in humans is encoded by the TFAP2A gene. It is mapped to 6p24.3. The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. TFAP2A acts as a sequence-specific DNA-binding transcription factor recognizing and binding to the specific DNA sequence and recruiting transcription machinery. This gene is expressed in neural crest cell lineages with the highest levels of expression corresponding to early neural crest cells, suggesting that TFAP2A plays a role in their differentiation and development.

Uniprot ID

P05549

文献引用格式

AP-2/TFAP2A Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0096)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

transcription factor AP-2 alpha (activating enhancer binding protein 2 alpha)

蛋白名全称

Transcription factor AP-2-alpha

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实验步骤

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