Anti-Alpha E-Catenin/CTNNA1 Antibody (Clone#10I2)

筛选器： All WB IHC FCM

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  Western blot analysis of Alpha E-Catenin/CTNNA1 using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: HEK293 whole cell lysates,
  Lane 3: U2OS whole cell lysates,
  Lane 4: U-87MG whole cell lysates,
  Lane 5: PC-12 whole cell lysates,
  Lane 6: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alpha E-Catenin/CTNNA1 antigen affinity purified monoclonal antibody (M01617-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha E-Catenin/CTNNA1 at approximately 100 kDa. The expected band size for Alpha E-Catenin/CTNNA1 is at 100 kDa.

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  IHC analysis of Alpha E-Catenin/CTNNA1 using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1).
  Alpha E-Catenin/CTNNA1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha E-Catenin/CTNNA1 Antibody (M01617-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  Flow Cytometry analysis of Jurkat cells using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1).
  Overlay histogram showing Jurkat cells stained with M01617-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-Alpha E-Catenin/CTNNA1 Antibody (M01617-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

• 

  Western blot analysis of Alpha E-Catenin/CTNNA1 using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: HEK293 whole cell lysates,
  Lane 3: U2OS whole cell lysates,
  Lane 4: U-87MG whole cell lysates,
  Lane 5: PC-12 whole cell lysates,
  Lane 6: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Alpha E-Catenin/CTNNA1 antigen affinity purified monoclonal antibody (M01617-1) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Alpha E-Catenin/CTNNA1 at approximately 100 kDa. The expected band size for Alpha E-Catenin/CTNNA1 is at 100 kDa.

• 

  IHC analysis of Alpha E-Catenin/CTNNA1 using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1).
  Alpha E-Catenin/CTNNA1 was detected in a paraffin-embedded section of human mammary cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Alpha E-Catenin/CTNNA1 Antibody (M01617-1) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

• 

  Flow Cytometry analysis of Jurkat cells using anti-Alpha E-Catenin/CTNNA1 antibody (M01617-1).
  Overlay histogram showing Jurkat cells stained with M01617-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with mouse anti-Alpha E-Catenin/CTNNA1 Antibody (M01617-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.