产品名称
Anti-TXNRD1 Antibody (Clone#OTI3E1)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
GRIM 12; GRIM12; KDRF; thioredoxin reductase 1; Thioredoxin reductase TR1; TR; TR1; TRXR1; TXNR; TXNRD1
免疫原
Full length human recombinant protein of human TXNRD1 (NP_003321) produced in E.coli.
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.
文献引用格式
TXNRD1 Antibody (Clone#OTI3E1) (Boster Biological Technology, Wuhan, China. Catalog#M01778-1)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
thioredoxin reductase 1
蛋白名全称
Thioredoxin reductase 1, cytoplasmic
推荐稀释比
Western blot (WB): | 1:500~2000 |