Anti-Cytokeratin 19/KRT19 Antibody (Clone#3D4)

筛选器： All WB IHC IF

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  Western blot analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human SK-OV-3 whole cell lysates,
  Lane 4: human COLO-320 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 19/KRT19 antigen affinity purified monoclonal antibody (M02101-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 19/KRT19 at approximately 44 kDa. The expected band size for Cytokeratin 19/KRT19 is at 44 kDa.

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  IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-2).
  Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Cytokeratin 19/KRT19 Antibody (M02101-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

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  IF analysis of Cytokeratin 19 using anti- Cytokeratin 19 antibody (M02101-2). Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti- Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. DyLight550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

• 

  Western blot analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human placenta tissue lysates,
  Lane 3: human SK-OV-3 whole cell lysates,
  Lane 4: human COLO-320 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with mouse anti-Cytokeratin 19/KRT19 antigen affinity purified monoclonal antibody (M02101-2) at a dilution of 1:1000 and probed with a goat anti-mouse IgG-HRP secondary antibody (Catalog # BA1050). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Cytokeratin 19/KRT19 at approximately 44 kDa. The expected band size for Cytokeratin 19/KRT19 is at 44 kDa.

• 

  IHC analysis of Cytokeratin 19/KRT19 using anti-Cytokeratin 19/KRT19 antibody (M02101-2).
  Cytokeratin 19/KRT19 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was incubated with mouse anti-Cytokeratin 19/KRT19 Antibody (M02101-2) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB (Catalog # AR1027) as the chromogen.

• 

  IF analysis of Cytokeratin 19 using anti- Cytokeratin 19 antibody (M02101-2). Cytokeratin 19 was detected in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL mouse anti- Cytokeratin 19 Antibody (M02101-2) overnight at 4°C. DyLight550 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:200 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.