Anti-Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8)

Mouse monoclonal antibody

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筛选器： All WB IHC ICC/IF

M02101-6

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT19 monoclonal antibody.
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT19 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT19(Cat# M02101-6).
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Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Carcinoma of kidney tissue using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-KRT19mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100°C for 10min, M02101-6, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.
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Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT19 mouse monoclonal antibody at 1:150 dilution.
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Anti-KRT19 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT19 .
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Cell and organelle markers; CK 19; CK19; Cytokeratin 19; Cytoskeleton Marker; K19; K1CS; keratin 19; KRT19

产品类型

Monoclonal

检验物种

human, rat, dog, monkey

应用范围

WB, IHC, ICC/IF

基因名称

KRT19

克隆号

OTI3F8

宿主

Mouse

抗体亚型

IgG1

免疫原

Recombinant fragment expressed in E.coli corresponding to amino acids 240-390 of human CK19

计算分子量

44 kDa

实际分子量

44 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

0.15 mg/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

Keratin, type I cytoskeletal 19 is a protein that in humans is encoded by the KRT19 gene.The protein encoded by this gene is a member of the keratin family. It is specifically expressed in the periderm, the transiently superficial layer that envelops the developing epidermis. The type I cytokeratins are clustered in a region of chromosome 17q12-q21. Due to its high sensitivity, KRT19 is the most used marker for the RT-PCR-mediated detection of tumor cells disseminated in lymph nodes, peripheral blood, and bone marrow of breast cancer patients.Keratin 19 is often used together with keratin 8 and keratin 18 to differentiate cells of epithelial origin from hematopoietic cells in tests that enumerate circulating tumor cells in blood.

Uniprot ID

P08727

文献引用格式

Cytokeratin 19/KRT19 Antibody (Clone#OTI3F8) (Boster Biological Technology, Wuhan, China. Catalog#M02101-6)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

keratin 19

蛋白名全称

Keratin, type I cytoskeletal 19

实验方案>

实验步骤

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