产品名称
Anti-VE-Cadherin/CDH5 DyLight 488 Conjugated Antibody
指标别称
7B4; 7B4 antigen; Cadherin 5; CD144; CDH5; FLJ17376; Vascular endothelial cadherin; VE cadherin
免疫原
E. coli-derived human VE Cadherin recombinant protein (Position: D48-R272).
激发/发射光谱
Amax=488nm; Emax=515-545nm
成分
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
纯化方式
Immunogen affinity purified.
保存条件
At -20˚C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
背景资料
CDH5 (Cadherin 5), also known as VE-cadherin, is a type of cadherin. It is encoded by the human gene CDH5. This gene is mapped to 16q22.1 using somatic cell hybrid panels. Functioning as a classic cadherin by imparting to cells the ability to adhere in a homophilic manner, the protein may play an important role in endothelial cell biology through control of the cohesion and organization of the intercellular junctions. Therefore it was concluded that VE-cadherin serves the purpose of maintaining newly formed vessels.
文献引用格式
VE-Cadherin/CDH5 DyLight 488 Conjugated Antibody (Boster Biological Technology, Wuhan, China. Catalog#M02632-Dyl488)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P).
推荐稀释比
Flow cytometry (FCM): | 1-3 μg/1x106 cells |