Western blot (WB): | 1:2000 |
Immunohistochemistry (IHC): | 1:150 |
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA02632)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH5(Cat# MA02632).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CDH5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH8.5, 120°C for 3min, MA02632)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CDH5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CDH5(Cat# MA02632).