Anti-Survivin/BIRC5 Antibody (Clone#OTI2D9)

Mouse monoclonal antibody

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MA00379

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期： 5-7天

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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, MA00379, Dilution 1:50)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-BIRC5 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris, pH9.0, 120°C for 3min, MA00379, Dilution 1:50)
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HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BIRC5 antibody, and then analyzed by flow cytometry.
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Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-BIRC5 monoclonal antibody at 1:200 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
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Immunofluorescent staining of 293T cells transfected by pCMV6-ENTRY BIRC5 using anti-BIRC5 antibody. 293T cells transfected with empty vector served as a negative control (MERGED, lower right) (1:100).
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BIRC5 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BIRC5 (Cat# MA00379).
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产品简介>

产品名称

Anti-Survivin/BIRC5 Antibody (Clone#OTI2D9)

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

API4; Apoptosis inhibitor 4; Apoptosis inhibitor survivin; BIRC5; EPR 1; IAP4; SURVIVIN

产品类型

Monoclonal

检验物种

human

应用范围

WB, ICC/IF, FCM

基因名称

BIRC5

克隆号

OTI2D9

宿主

Mouse

抗体亚型

IgG1

免疫原

Full length human recombinant protein of human BIRC5 (NP_001159) produced in HEK293T cell.

计算分子量

16 kDa

实际分子量

16.2 kDa

成分

PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

纯化方式

Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)

浓度

500 ug/ml

产品形态

Liquid

保存条件

Stable for 12 months from date of receipt. Store at -20°C as received.

背景资料

This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined.

Uniprot ID

O15392

文献引用格式

Survivin/BIRC5 Antibody (Clone#OTI2D9) (Boster Biological Technology, Wuhan, China. Catalog#MA00379)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

baculoviral IAP repeat containing 5

实验方案>

实验步骤

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