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Anti-MMP13 Antibody (Clone#OTI3H3)

Mouse monoclonal antibody

说明书

筛选器: All IHC ICC/IF WB

MA00420

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Figure 1. Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00420)

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  • Figure 2. Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00420)

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  • Figure 3. Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-MMP13 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA00420)

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  • Figure 4. Anti-MMP13 mouse monoclonal antibody (MA00420) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MMP13 .

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  • Figure 5. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MMP13 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MMP13.

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产品名称
Anti-MMP13 Antibody (Clone#OTI3H3)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CLG3; Matrix metalloproteinase 13; MMP 13; MMP13
产品类型
Monoclonal
检验物种
human, mouse, rat
应用范围
WB, IHC, ICC/IF
基因名称
MMP13
克隆号
OTI3H3
抗体来源
Mouse
抗体亚型
IgG1
免疫原
Human recombinant protein fragment corresponding to amino acids 104-471 of human MMP13(NP_002418) produced in HEK293T cell.
计算分子量
54 kDa
实际分子量
42.2 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
Collagenase 3 is an enzyme that in humans is encoded by the MMP13 gene. This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This protease cleaves type II collagen more efficiently than types I and III. It may be involved in articular cartilage turnover and cartilage pathophysiology associated with osteoarthritis. Mutations in this gene are associated with metaphyseal anadysplasia. This gene is part of a cluster of MMP genes on chromosome 11.
Uniprot ID
P45452  
文献引用格式
MMP13 Antibody (Clone#OTI3H3) (Boster Biological Technology, Wuhan, China. Catalog#MA00420)
应用释义
WB- 蛋白质免疫印迹法,IHC- 免疫组织化学法,ICC/IF-免疫细胞荧光,ICC-免疫细胞化学,IHC-F- 冰冻切片免疫组化,FCM-流式细胞术,ELISA-酶联免疫吸附测定,IP-免疫沉淀法 ,IF-免疫组织荧光法,ChIP-染色质免疫沉淀法
基因名全称
matrix metallopeptidase 13
蛋白名全称
Collagenase 3
推荐稀释比
Western blot (WB):1:1000
Immunohistochemistry (IHC):1:150
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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    Anti-MMP13 Antibody (Clone#OTI3H3)

    筛选器: All IHC ICC/IF WB

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