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Anti-Chromogranin A/CHGA Antibody (Clone#OTI2F2)

Mouse monoclonal antibody

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MA01256

  • 50μl ¥1280 100μl ¥2180 150μl ¥2800
  • 货期: 5-7天
  • Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-CHGA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min, MA01256)

    all(3)
  • Immunofluorescent staining of HeLa cells using anti-CHGA mouse monoclonal antibody.

    all(3)
  • HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHGA (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHGA.

    all(3)

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产品名称
Anti-Chromogranin A/CHGA Antibody (Clone#OTI2F2)
规格/价格
50μl/1280 100μl/2180 150μl/2800
指标别称
CGA; CHGA; Chromogranin A; Pituitary secretory protein I; SP I; Vasostatin I Vasostatin 2
产品类型
Monoclonal
检验物种
human
应用范围
WB, IHC, ICC/IF
基因名称
CHGA
克隆号
OTI2F2
宿主
Mouse
抗体亚型
IgG1
免疫原
Full length human recombinant protein of human CHGA(NP_001266) produced in HEK293T cell.
实际分子量
70-80 kDa
成分
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
纯化方式
Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
浓度
500 ug/ml
产品形态
Liquid
保存条件
Stable for 12 months from date of receipt. Store at -20°C as received.
背景资料
The protein encoded by this gene is a member of the chromogranin/secretogranin family of neuroendocrine secretory proteins. It is found in secretory vesicles of neurons and endocrine cells. This gene product is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. However, biological activities for these molecules have not been shown. [provided by RefSeq, Jul 2008]
Uniprot ID
P10354  
文献引用格式
Chromogranin A/CHGA Antibody (Clone#OTI2F2) (Boster Biological Technology, Wuhan, China. Catalog#MA01256)
应用释义
WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
基因名全称
chromogranin A
蛋白名全称
Chromogranin-A
推荐稀释比
Western blot (WB):1:4000
Immunohistochemistry (IHC):1:500
Immunocytochemistry/Immunofluorescence (ICC/IF):1:100

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