Anti-c-Cbl/CBL Antibody

筛选器： All WB FCM

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  Figure 1. Western blot analysis of c-Cbl/CBL using anti-c-Cbl/CBL antibody (PB0022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Mcf-7 whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: A549 whole cell lysates,
  Lane 5: RH35 whole cell lysates,
  Lane 6: Ana-1 whole cell lysates,
  Lane 7: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Cbl/CBL antigen affinity purified polyclonal antibody (PB0022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Cbl/CBL at approximately 120 kDa. The expected band size for c-Cbl/CBL is at 100 kDa.

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  Figure 6. Flow Cytometry analysis of A549 cells using anti-c-Cbl/CBL antibody (PB0022).
  Overlay histogram showing A549 cells stained with PB0022 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Cbl/CBL Antibody (PB0022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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  Figure 1. Western blot analysis of c-Cbl/CBL using anti-c-Cbl/CBL antibody (PB0022). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: Hela whole cell lysates,
  Lane 2: Mcf-7 whole cell lysates,
  Lane 3: HepG2 whole cell lysates,
  Lane 4: A549 whole cell lysates,
  Lane 5: RH35 whole cell lysates,
  Lane 6: Ana-1 whole cell lysates,
  Lane 7: NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Cbl/CBL antigen affinity purified polyclonal antibody (PB0022) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Cbl/CBL at approximately 120 kDa. The expected band size for c-Cbl/CBL is at 100 kDa.

• 

  Figure 6. Flow Cytometry analysis of A549 cells using anti-c-Cbl/CBL antibody (PB0022).
  Overlay histogram showing A549 cells stained with PB0022 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-c-Cbl/CBL Antibody (PB0022) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.