Anti-c-Cbl/CBL Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB

BA3809-2

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of c-Cbl/CBL using anti-c-Cbl/CBL antibody (BA3809-2). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: MCF-7 whole cell lysates,
Lane 2: RH35 whole cell lysates,
Lane 3: Ana-1 whole cell lysates,
Lane 4: NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-c-Cbl/CBL antigen affinity purified polyclonal antibody (BA3809-2) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for c-Cbl/CBL at approximately 120 kDa. The expected band size for c-Cbl/CBL is at 100 kDa.
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产品名称

Anti-c-Cbl/CBL Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

C CBL; CBL; CBL2; c-Cbl; Proto oncogene c Cbl; RING finger protein 55; RNF55

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

CBL

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human CBL, identical to the related rat and mouse sequences.

计算分子量

100 kDa

实际分子量

120 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

CBL(Cbl proto-oncogene) is also known as C-CBL, RNF55, CBL2 and E3 ubiquitin protein ligase. Wei et al.(1990) mapped the CBL gene to chromosome 11q23.3-qter by molecular characterization of the breakpoints in 2 somatic cell hybrids. The encoded protein is one of the enzymes required for targeting substrates for degradation by the proteasome. This protein mediates the transfer of ubiquitin from ubiquitin conjugating enzymes(E2) to specific substrates. This protein also contains an N-terminal phosphotyrosine binding domain that allows it to interact with numerous tyrosine-phosphorylated substrates and target them for proteasome degradation. As such it functions as a negative regulator of many signal transduction pathways. This gene has been found to be mutated or translocated in many cancers including acute myeloid leukaemia. Mutations in this gene are also the cause of Noonan syndrome-like disorder.

Uniprot ID

P22681

文献引用格式

c-Cbl/CBL Antibody (Boster Biological Technology, Wuhan, China. Catalog#BA3809-2)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Cbl proto-oncogene, E3 ubiquitin protein ligase

蛋白名全称

E3 ubiquitin-protein ligase CBL

实验方案>

实验步骤

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