Anti-HPSE Antibody

Rabbit polyclonal antibody

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筛选器： All WB

PB0405

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of HPSE using anti-HPSE antibody (PB0405). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: A431 whole cell lysates,
Lane 2: U87 whole cell lysates,
Lane 3: HELA whole cell lysates,
Lane 4: U2OS whole cell lysates,
Lane 5: THP-1 whole cell lysates,
Lane 6: MCF-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HPSE antigen affinity purified polyclonal antibody (PB0405) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HPSE at approximately 65 kDa. The expected band size for HPSE is at 61 kDa.
all(2)
Western blot analysis of HPSE using anti-HPSE antibody (PB0405). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: rat ovary tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse lung tissue lysates,
Lane 5: mouse testis tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-HPSE antigen affinity purified polyclonal antibody (PB0405) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for HPSE at approximately 65 kDa. The expected band size for HPSE is at 61 kDa.
all(2)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-HPSE Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Endo glucoronidase; HEP; heparanase; Heparanase 1; HPA; Hpa1; HPR1; HPSE; HPSE1; HSE1

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

基因名称

HPSE

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence in the middle region of human Heparanase 1 different from the related mouse and rat sequences by eight amino acids.

计算分子量

61 kDa

实际分子量

65 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

Heparanase, also known as HPSE, is an enzyme that acts both at the cell-surface and within the extracellular matrix to degrade polymeric heparan sulfate molecules into shorter chain length oligosaccharides. Heparanase is an endo-beta-D-glucuronidase capable of cleaving heparan sulfate and has been implicated in inflammation and tumor angiogenesis and metastasis. The successful penetration of the endothelial cell layer that lines the interior surface of blood vessels is an important process in the formation of blood borne tumour metastases. Heparan sulfate proteoglycans are major constituents of this layer and it has been shown that increased metastatic potential corresponds with increased heparanase activity for a number of cell lines.

Uniprot ID

Q9Y251

文献引用格式

HPSE Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0405)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

heparanase

蛋白名全称

Heparanase

实验方案>

实验步骤

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