Anti-PDGFR alpha/PDGFRA Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0822

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of PDGFR alpha/PDGFRA using anti-PDGFR alpha/PDGFRA antibody (PB0822). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HT1080 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human U2OS whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-PDGFR alpha/PDGFRA antigen affinity purified polyclonal antibody (PB0822) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for PDGFR alpha/PDGFRA at approximately 180 kDa. The expected band size for PDGFR alpha/PDGFRA is at 123 kDa.
all(2)
IHC analysis of PDGFR alpha/PDGFRA using anti-PDGFR alpha/PDGFRA antibody (PB0822).
PDGFR alpha/PDGFRA was detected in a paraffin-embedded section of human colon cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-PDGFR alpha/PDGFRA Antibody (PB0822) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(2)

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产品简介>

产品名称

Anti-PDGFR alpha/PDGFRA Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

platelet derived growth factor receptor alpha; CD140A; PDGFR-2; PDGFR2; PDGFRA

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

PDGFRA

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human PDGFRA identical to the related mouse sequence, and different from the related rat sequence by one amino acid.

计算分子量

123 kDa

实际分子量

180 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

PDGFRA (Platelet-derived growth factor receptor, alpha), also called PDGFR2, encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. The PDGFA gene is mapped on 4q12. The PDGFRA-FIP1L1 gene is a constitutively activated tyrosine kinase that transforms hematopoietic cells and is a therapeutic target of imatinib. And the PDGFRA gene contains 23 exons spanning about 65 kb. Using the human PDGFRA promoter linked to a luciferase reporter, Joosten et al. showed that PAX1 acts as a transcriptional activator of the PDGFRA gene in differentiated human embryonal carcinoma cells. PDGFRA is responsible for mediating cellular contraction of multiple growth factors: TGFB1 and members of the PDGF family. Lei et al. noted that in the rabbit model of the disease, PDGFRA is dramatically more capable of promoting PVR than is the closely related PDGFRB. PDGFRA is a critical receptor required for human CMV infection, and thus a target for novel antiviral therapies.

Uniprot ID

P16234

文献引用格式

PDGFR alpha/PDGFRA Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0822)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

platelet-derived growth factor receptor, alpha polypeptide

蛋白名全称

Platelet-derived growth factor receptor alpha

实验方案>

实验步骤

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