Anti-Collagen Type I/COL1A1 Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB0981

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of Collagen Type I/COL1A1 using anti-Collagen Type I/COL1A1 antibody (PB0981). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Collagen Type I/COL1A1 antigen affinity purified polyclonal antibody (PB0981) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Collagen Type I/COL1A1 at approximately 130,180-200. The expected band size for Collagen Type I/COL1A1 is at 138 kDa.
all(3)
IHC analysis of Collagen Type I/COL1A1 using anti-Collagen Type I/COL1A1 antibody (PB0981).
Collagen Type I/COL1A1 was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Collagen Type I/COL1A1 Antibody (PB0981) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)
IHC analysis of Collagen Type I/COL1A1 using anti-Collagen Type I/COL1A1 antibody (PB0981).
Collagen Type I/COL1A1 was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-Collagen Type I/COL1A1 Antibody (PB0981) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(3)

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产品名称

Anti-Collagen Type I/COL1A1 Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

Alpha 1 type I collagen; COL1A1; Collagen alpha 1(I) chain; collagen; type I; alpha 1; OI4

产品类型

Polyclonal

检验物种

mouse, rat

应用范围

WB, IHC

基因名称

COL1A1

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I different from the related human sequence by three amino acids, and identical to the related rat sequence.

计算分子量

138 kDa

实际分子量

130,180-200

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

Uniprot ID

P11087

文献引用格式

Collagen Type I/COL1A1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB0981)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Collagen alpha-1(I) chain

蛋白名全称

Collagen alpha-1(I) chain

注意事项

WB实验中，COL1A1在样本中存在很多的剪切位点，如果样本处理不当，很容易发生降解或是剪切，因此建议使用新鲜制备的样本，同时提高裂解液中蛋白酶抑制剂的浓度，最大程度避免蛋白发生降解。如果组织样本常规提取的蛋白检测不到或检测条带很弱，可以尝试用以下方式制备样本：
1．取组织，剔去组织外部脂肪及结缔组织。
2．丙酮中浸泡过夜，进一步去除残留脂肪，用冷蒸馏水清洗，除去丙酮（若组织本身不含脂肪，可以省略此步）。
3．将组织切碎，倒入少量0.5mol/L乙酸（pH2.5），用打碎机打制成匀浆，加入胃蛋白酶。酶量与湿组织量之比为1:100（g/ml），再加入足量0.5mol/L乙酸，使溶液中酶的终浓度为2mg/ml。搅拌过夜（建议48小时）。16,000r、45min离心。
4．留上清。沉淀部分重复第3步，弃去沉淀。将2次上清合并。
5．上清边搅拌边缓缓加入氯化钠粉末，使终浓度至0.7mol/L（1 L溶液中含有40.95 g氯化钠），放置过夜。16,000r、45min离心，弃上清。
6. 沉淀用匀浆器匀浆，加0.5mol/L乙酸溶液，使溶液完全透明。
7．边搅拌边缓慢加入氯化钠，使终浓度为0.7mol/L，放置过夜。
8．16,000r×45min离心，弃上清。沉淀即为组织胶原。
9. 沉淀用2M尿素溶解，再加等体积的2X上样缓冲液混匀，置100℃水浴箱煮沸变性5分钟，至此样本制备完成。

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