Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse ANA-1 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates,
Lane 10: mouse Neuro-2a whole cell lysates,
Lane 11: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (PB9341) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis using anti- NPM1 antibody (PB9341). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
all(7) 
Figure 6. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of HEL cells using anti-B23/NPM1 antibody (PB9341).
Overlay histogram showing HEL cells stained with PB9341 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse ANA-1 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates,
Lane 10: mouse Neuro-2a whole cell lysates,
Lane 11: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (PB9341) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis using anti- NPM1 antibody (PB9341). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
Figure 6. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of HEL cells using anti-B23/NPM1 antibody (PB9341).
Overlay histogram showing HEL cells stained with PB9341 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human MCF7 whole cell lysates,
Lane 4: rat C6 whole cell lysates,
Lane 5: rat NRK whole cell lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: rat RH35 whole cell lysates,
Lane 8: mouse ANA-1 whole cell lysates,
Lane 9: mouse RAW264.7 whole cell lysates,
Lane 10: mouse Neuro-2a whole cell lysates,
Lane 11: mouse Hepa1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (PB9341) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of mouse intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of rat intestine tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis using anti- NPM1 antibody (PB9341). detected in paraffin-embedded section of human intestinal cancer tissue. The tissue section were stained using the Dylight550-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog # BA1135) and counterstained with DAPI (blue).
Figure 6. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (PB9341).
B23/NPM1 was detected in an immunocytochemical section of Caco-2 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 7. Flow Cytometry analysis of HEL cells using anti-B23/NPM1 antibody (PB9341).
Overlay histogram showing HEL cells stained with PB9341 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-B23/NPM1 Antibody (PB9341) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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