Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: Rat Brain tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. ICC analysis of B23/NPM1 using anti- B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence(ICC/IF): | 1:50-400 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: Rat Brain tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. ICC analysis of B23/NPM1 using anti- B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
Figure 1. Western blot analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat Testis tissue lysates,
Lane 2: Rat Brain tissue lysates,
Lane 3: HELA whole cell lysates,
Lane 4: U87 whole cell lysates,
Lane 5: A549 whole cell lysates,
Lane 6: SMMC whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-B23/NPM1 antigen affinity purified polyclonal antibody (BA3863) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for B23/NPM1 at approximately 38-40 kDa. The expected band size for B23/NPM1 is at 33 kDa.
Figure 2. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of human intestinal cancer tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. ICC analysis of B23/NPM1 using anti- B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of Hela cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in a paraffin-embedded section of rat spleen tissue. The tissue section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IF analysis of B23/NPM1 using anti-B23/NPM1 antibody (BA3863).
B23/NPM1 was detected in an immunocytochemical section of A431 cells. The section was incubated with rabbit anti-B23/NPM1 Antibody (BA3863) at a dilution of 1:100. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody.
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