Anti-RAGE/AGER Antibody

Rabbit polyclonal antibody

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筛选器： All WB IHC

PB9469

50μl ￥1280 100μl ￥2180 150μl ￥2800
货期：现货

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Western blot analysis of RAGE/AGER using anti-RAGE/AGER antibody (PB9469). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat lung tissue lysates,
Lane 2: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-RAGE/AGER antigen affinity purified polyclonal antibody (PB9469) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for RAGE/AGER at approximately 45-58 kDa. The expected band size for RAGE/AGER is at 43 kDa.
all(4)
IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (PB9469).
RAGE/AGER was detected in a paraffin-embedded section of mouse lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAGE/AGER Antibody (PB9469) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (PB9469).
RAGE/AGER was detected in a paraffin-embedded section of rat lung tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAGE/AGER Antibody (PB9469) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)
IHC analysis of RAGE/AGER using anti-RAGE/AGER antibody (PB9469).
RAGE/AGER was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-RAGE/AGER Antibody (PB9469) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
all(4)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-RAGE/AGER Antibody

规格/价格

50μl/1280 100μl/2180 150μl/2800

指标别称

AGER; RAGE

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC

基因名称

AGER

宿主

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human RAGE, different from the related mouse and rat sequences by six amino acids.

计算分子量

43 kDa

实际分子量

45-58 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied.

背景资料

The receptor for advanced glycation end products (RAGE) is a multi-ligand member of the immunoglobulin superfamily of cell surface molecules. It interacts with distinct molecules implicated in homeostasis, development and inflammation, and certain diseases such as diabetes and Alzheimer's disease. RAGE is also a central cell surface receptor for amphoterin and EN-RAGE. And RAGE is associated with sustained NF-kappaB activation in the diabetic microenvironment and has a central role in sensory neuronal dysfunction. Moreover, RAGE propagates cellular dysfunction in several inflammatory disorders and diabetes, and it also functions as an endothelial adhesion receptor promoting leukocyte recruitment.

Uniprot ID

Q15109

RRID

AB_3082129

文献引用格式

RAGE/AGER Antibody (Boster Biological Technology, Wuhan, China. Catalog#PB9469)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光和免疫细胞化学; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

advanced glycosylation end product-specific receptor

蛋白名全称

Advanced glycosylation end product-specific receptor

实验方案>

实验步骤

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