Western blot (WB): | 1:500-2000 |
Immunohistochemistry (IHC): | 1:50-400 |
Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
(Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
Western blot analysis of BMP2 using anti-BMP2 antibody (PB9687). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Rat brain tissue lysates,
Lane 3: U87 whole cell lysates,
Lane 4: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP2 antigen affinity purified polyclonal antibody (PB9687) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP2 at approximately 20 kDa, 40 kDa. The expected band size for BMP2 is at 45 kDa.
IHC analysis of BMP2 using anti-BMP2 antibody (PB9687).
BMP2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BMP2 Antibody (PB9687) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.
Western blot analysis of BMP2 using anti-BMP2 antibody (PB9687). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: Rat lung tissue lysates,
Lane 2: Rat brain tissue lysates,
Lane 3: U87 whole cell lysates,
Lane 4: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BMP2 antigen affinity purified polyclonal antibody (PB9687) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BMP2 at approximately 20 kDa, 40 kDa. The expected band size for BMP2 is at 45 kDa.
IHC analysis of BMP2 using anti-BMP2 antibody (PB9687).
BMP2 was detected in a paraffin-embedded section of human intestinal cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was incubated with rabbit anti-BMP2 Antibody (PB9687) at a dilution of 1:200 and developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB (Catalog # AR1027) as the chromogen.