Anti-GAPDH Antibody (HRP)

Rabbit polyclonal antibody

说明书

筛选器： All WB

A00227-HRP

100μl ￥880 200μl ￥1680
货期：现货

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Figure 1. Western blot analysis of anti-GAPDH antibody (A00227-HRP). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human A562 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat liver tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified polyclonal antibody (A00227-HRP). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-GAPDH Antibody (HRP)

规格/价格

100μl/880 200μl/1680

指标别称

G3PD; GAPD; GAPDH; OK/SW cl.12

产品类型

Polyclonal

检验物种

human, mouse, rat, monkey, zebrafish, chicken

应用范围

基因名称

GAPDH

抗体来源

Rabbit

抗体亚型

IgG

免疫原

Polypeptide

计算分子量

36 kDa

成分

Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.

纯化方式

HRP-GAPDH antibody is affinity chromatography purified via peptide column.

浓度

0.5 mg/mL

产品形态

Liquid

保存条件

HRP-GAPDH antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

背景资料

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. It also is involved in a number of cellular processes such as membrane fusion, phosphotransferase activity, DNA replication and repair, and nuclear RNA export (1). GAPDH also plays a role in different pathologies such as cancer progression, apoptosis, and neuronal diseases such as Alzheimer's and Huntington's disease (2). GAPDH is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.

Uniprot ID

P04406

文献引用格式

GAPDH Antibody (HRP) (Boster Biological Technology, Wuhan, China. Catalog#A00227-HRP)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

Glyceraldehyde-3-phosphate dehydrogenase

蛋白名全称

Glyceraldehyde-3-phosphate dehydrogenase

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实验步骤

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