Western blot analysis of different concentration of Anti-Flag Tag(1E6) Monoclonal Antibody (M30971)
Blocking: Incubate the membrane with 5% Non-Fat Milk, PBS, 4 ⊥ overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, TBS, 0.1% Tween-20, RT, 30mins

Western blot analysis of different concentration of Anti-GFP (8C1) Monoclonal Antibody
Antigen: Extracts from E.coli expressing GFP-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL

Western blot analysis of different concentration of Anti-GST Tag (5A7) Monoclonal Antibody
Antigen: Extracts from E.coli expressing GST-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL

Western blot analysis of different concentration of Anti-HA Tag(1A4) Monoclonal Antibody (M30974)
Blocking: Incubate the membrane with 5% Non-Fat Milk, 1PBS, 4°C, overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1TBS, 0.1% Tween-20, RT, 30mins

Western blot analysis of different concentration of Anti- His Tag (19b10) Monoclonal Antibody (M30975-1)Blocking: Incubate the membrane with 5% Non-Fat Milk, 1×PBS, 4℃, overnightAntibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1×TBS, 0.1% Tween-20, RT, 30mins.

Western blot analysis of different concentration of Anti-His tag (9C11) Monoclonal Antibody
Antigen: Extracts from E.coli expressing His-tagged protein
Line No.
1: 1 ug/mL
2: 0.5 ug/mL
3: 0.1 ug/mL

Western blot analysis of different concentration of Anti-Myc Tag(10D11) Monoclonal Antibody (M00026-2)
Blocking: Incubate the membrane with 5% Non-Fat Milk, 1PBS, 4 overnight
Antibody Incubation: Diluted antibody in 5% Non-Fat Milk, 1TBS, 0.1% Tween-20, RT, 30mins

Western blot analysis of extracts from V5-tag fusion protein, using V5-tag Mouse Monoclonal Antibody.