Anti-AATF (Phospho-Ser477) Antibody

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  Western blot of HeLa cell lysate showing specific immunolabeling of the ~66 kDa Che-1 protein phosphorylated at Ser⁴⁷⁷ in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is blocked by preadsorption of the phosphopeptide used as the antigen, but not by the corresponding non-phosphopeptide (not shown).

Anti-ABL1 (Phospho-T735) Antibody

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  Western blot analysis of lysates from COS7 cells treated with EGF 200ng/ml 30', using ABL1 (Phospho-Thr735) Antibody. The lane on the right is blocked with the phospho peptide.

Anti-ABL1 (Phospho-Y204) Antibody

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  Figure 1. Immunohistochemistry validation of ABL1 using Anti-Phospho-Abl1 (Y204) Antibody (A00133Y204).

  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C

Anti-ABL1 (Phospho-Y245) Antibody

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  Western blot analysis of lysates from K562 cells treated with Insulin 0.01U/ml 15, using c-Abl (Phospho-Tyr245) Antibody. The lane on the right is blocked with the phospho peptide.

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  Immunofluorescence analysis of HeLa cells, using c-Abl (Phospho-Tyr245) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-ABL1/2 (Phospho-Tyr393/439) Antibody

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  Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ABL1/2(Phospho-Tyr393/439) antibody P00133(left) or the same antibody preincubated with blocking peptide(right).

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  Western blot analysis of extracts from HL60 cells using ABL1/2(phospho-Tyr393/439) antibody P00133 and the same antibody preincubated with blocking peptide.

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  Immunofluorescence staining of methanol-fixed Hela cells using ABL1/2(phospho-Tyr393/439) antibody P00133.

Anti-ACC1/ACACA(Phospho-S79) Antibody

筛选器： All WB

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  Western blot analysis of Phospho-ACC(S79) expression in (1) A431 cell lysate; (2) A431 cell treated with lambda phosphatase lysate.

Anti-AKT1 (Phospho-S124) Antibody

筛选器： All WB IP

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  Western blot analysis of Phospho-AKT1 (S124) expression in (1) MCF-7 cell treated with Alkaline Phosphatase lysate; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AKT1 (Phospho-S129) Antibody

筛选器： All WB

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  Western blot analysis of Phospho-AKT1 (S129) expression in (1) MCF-7 cell lysate treated with Alkaline Phosphatase; (2) MCF-7 cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AKT1 (Phospho-T450) Antibody

筛选器： All WB IHC ICC/IF IP

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  Figure 1. Western blot analysis of anti-AKT1 antibody (BM4721). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human MCF-7 whole cell lysates,
  Lane 3: rat brain tissue lysates,
  Lane 4: rat PC-12 whole cell lysates,
  Lane 5: mouse brain tissue lysates,
  Lane 6: mouse NIH/3T3 whole cell lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-AKT1 antigen affinity purified monoclonal antibody (BM4721) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for AKT1 at approximately 65 kDa. The expected band size for AKT1 is at 56 kDa.

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  Figure 2. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human intestinal diffuse large B-cell lymphoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 4. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 5. IHC analysis of AKT1 using anti-AKT1 antibody (BM4721) .
  AKT1 was detected in a paraffin-embedded section of human prostate cancer tissue. The tissue section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 6. IF analysis of AKT1 using anti-AKT1 antibody (BM4721) and anti-Beta Tubulin antibody (M01857-3).
  AKT1 was detected in an immunocytochemical section of A549 cells. The section was incubated with rabbit anti-AKT1 Antibody (BM4721) at a dilution of 1:100. Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and Cy3-conjugated Anti-mouse IgG Secondary Antibody (red)(Catalog#BA1031) were used as secondary antibody.

Anti-AKT1/3 (Phospho-Y437/434) Antibody

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  Figure 1. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western Blot (WB) analysis of 293T and HeLa cells using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody.
  Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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  Figure 2. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western Blot (WB) analysis of 293T cells using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody.
  Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration

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  Figure 3. Western blotting validation for Anti-Phospho-Akt1/3 (Y437/434) Antibody A00024Y437

  Western blot (WB) analysis of 293T

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  Figure 4. Immunohistochemistry validation of AKT1 using Anti-Phospho-Akt1/3 (Y437/434) Antibody (A00024Y437).

  Immunohistochemical analysis of paraffin-embedded Human brain using Anti-Phospho-Akt1/3 (Y437/434) polyclonal antibody. Antibody was diluted at 1:100 (4°C

Anti-AMPK Alpha 1/PRKAA1 (Phospho-S496) Antibody

筛选器： All WB ICC/IF IP

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  Western blot analysis of Phospho-AMPK alpha 1 (S496) expression in (1) 293T cell lysate treated with LP; (2) 293T cell lysate.

Anti-AMPK Alpha 2/PRKAA2 (Phospho-S345) Antibody

筛选器： All WB

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  Western blot analysis of Phospho-AMPK alpha 2 (S345) expression in (1) 293T cell lysate treated with Lambda Phosphatase; (2) 293T cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-AMPK Alpha 2/PRKAA2 (Phospho-S491) Antibody

筛选器： All WB

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  Western blot analysis of AMPK beta 1 expression in (1) 293T cell lysate treated with Lambda lysate; (2) 293T cell lysate.

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  All lanes use the Antibody for 1 hour at room temperature.

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  All lanes use the Antibody for 1 hour at room temperature.

Anti-APC1/ANAPC1 (Phospho S355) Antibody

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  Figure 1. Western blot analysis of ANAPC1, TSG24 using anti-ANAPC1, TSG24 antibody (P03471-2).
  Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
  After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ANAPC1, TSG24 antigen affinity purified polyclonal antibody (Catalog # P03471-2) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for ANAPC1, TSG24.

Anti-APC1/ANAPC1 (Phospho-S355) Antibody

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  Western blot analysis of phosphorylated APC1 expression in lysate from asynchronous cells (lane 1), lysate from cells treated with thymidine to synchronize cells at the G1/S boundary (lane 2) and lysate from cells treated with nocodazole to synchronize cells at the M phase (lane 3). Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Phosphorylated APC1 at 215KD was detected using rabbit anti-APC1 pS355 Antigen Affinity purified polyclonal antibody (Catalog # P03471) at 1:500. Data contributed by Bing Li, UT Southwestern.

Anti-APC1/ANAPC1 (Phospho-S355) Antibody

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  Figure 1. Immunohistochemistry validation of ANAPC1 using Anti-Phospho-APC1 (S355) ANAPC1 Antibody (A03471S355).

  Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100 (4°C