Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BM3873). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hacat whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human Hela whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse spleen tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified monoclonal antibody (BM3873) at a dilution of 1:2000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of anti- β-Actin antibody (BM0627).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Human HELA whole cell lysates,Lane 2: Human HepG2 whole cell lysates,Lane 3: Monkey kidney tissue lysates,Lane 4: Monkey liver tissue lysates,Lane 5: Rat brain tissue lysates,Lane 6: Rat PC-12 whole cell lysates,Lane 7: Mouse brain tissue lysates,Lane 8: Mouse ANA-1 whole cell lysates,Lane 9: Rabbit intestines tissue lysates,Lane 10: Rabbit intestines tissue lysates,Lane 11: Pig intestines tissue lysates.Use rabbit anti- β-Actin 1:5000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for β-Actin at approximately 42KD. The expected band size for β-Actin is at 42KD.

Figure 2.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Mouse Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 3.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Rat Intestine tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 4.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Intestinal Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 5.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Lung Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 6.IHC analysis using anti- β-Actin antibody (BM0627).detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1021) with DAB as the chromogen.

Figure 1. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human Jurkat whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse kidney tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 2. Western blot analysis of anti-Beta Actin/ACTB antibody (BA2305). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: zebra fish tissue lysates,
Lane 2: chicken heart tissue lysates,
Lane 3: chicken liver tissue lysates,
Lane 4: chicken brain tissue lysates,
Lane 5: monkey heart tissue lysates,
Lane 6: monkey lung tissue lysates,
Lane 7: monkey kidney tissue lysates,
Lane 8: monkey liver tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Actin/ACTB antigen affinity purified polyclonal antibody (BA2305) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Actin/ACTB at approximately 42 kDa. The expected band size for Beta Actin/ACTB is at 42 kDa.

Figure 1. Western blot analysis of anti- GAPDH antibody (A00227-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: Hela whole cell lysates,
Lane 2: Caco-2 whole cell lysates,
Lane 3: CCRF-CEM whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: rat liver tissue lysates,
Lane 6: mouse brain tissue lysates,
Lane 7: mouse liver tissue lysates.
Use rabbit anti- GAPDH 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for GAPDH at approximately 36KD. The expected band size for GAPDH is at 36KD.

Figure 2. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human Laryngeal squamous cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 3. IHC analysis using anti- GAPDH antibody (A00227-1).detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human A549 whole cell lysates,
Lane 5: human PC-3 whole cell lysates,
Lane 6: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Figure 2. Western blot analysis of anti-GAPDH antibody (BM3874). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: rat RH-35 whole cell lysates,
Lane 4: mouse liver tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM3874) at a dilution of 1:5000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Immunohistochemical analysis of paraffin-embedded human bladder cancer, using GAPDH Antibody.

Figure 1. Western blot analysis of anti-GAPDH antibody (BM1623). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: rat brain lysates,
Lane 6: rat RH-35 whole cell lysates,
Lane 7: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-GAPDH antigen affinity purified monoclonal antibody (BM1623) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for GAPDH at approximately 36 kDa. The expected band size for GAPDH is at 36 kDa.

Western blot analysis of alpha Tubulin expression in Saccharomyces cerevisiae (yeast) cell lysate.

alpha Tubulin(BM3885)(MW:55KD)大鼠脑，大鼠脑，小鼠脑，小鼠脑组织，人Hela细胞裂解，免疫印迹分析。

Immunohistochemical analysis of paraffin-embedded human bladder, using alpha Tubulin Antibody.

Figure 1. Western blot analysis of anti- alpha Tubulin antibody (BM1452). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: SH-SY5Y whole cell lysates,
Lane 2: Jurkat whole cell lysates,
Lane 3: A549 whole cell lysates,
Lane 4: rat brain tissue lysates,
Lane 5: mouse brain tissue lysates,
Lane 6: mouse thymus tissue lysates.
Use mouse anti- alpha Tubulin 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for alpha Tubulin at approximately 55KD. The expected band size for alpha Tubulin is at 50KD.

Figure 2. IHC analysis using anti- alpha Tubulin antibody (BM1452). detected in paraffin-embedded section of Human Mammary Cancer tissue. Biotinylated goat anti-mouse IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.

Figure 1. Western blot analysis of anti- ATP1A1 antibody (PB0504).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysates,Lane 2: human U87-MG whole cell lysates,Lane 3: human HEK293 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: human HEPG2 whole cell lysates,Lane 6: human SW620 whole cell lysates,Lane 7: monkey COS-7 whole cell lysates,Lane 8: human K562 whole cell lysates.Use rabbit anti- ATP1A1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ATP1A1 at approximately 100-113KD. The expected band size for ATP1A1 at 113KD.

Immunohistochemical analysis of paraffin-embedded Rat heart, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle - gastrocnemius , using the Antibody.

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using Sodium Potassium ATPase Antibody.

Immunohistochemical analysis of paraffin-embedded Human thyroid cancer, using the Antibody.

Immunohistochemical analysis of paraffin-embedded Human cervical cancer, using the Antibody.

Immunofluorescent analysis using the Antibody.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

All lanes use the Antibody for 1 hour at room temperature.

Figure 1. Western blot analysis of anti-BCL2A1 antibody (BM4421). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-BCL2A1 antigen affinity purified monoclonal antibody (BM4421) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for BCL2A1 at approximately 27 kDa. The expected band size for BCL2A1 is at 20 kDa.

All lanes use the Antibody for 1 hour at room temperature.

Western blot analysis of beta Actin expression in (1) Hela; (2)Human fetal kidney lysate; (3) 3T3 cell lysate; (4) PC-12 cell lysate; (5) COS-1 cell lysate; (6) Goat muscle lysate.

Western blot analysis of beta Actin expression in (1) Hela cell lysate, (2) Human liver lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) PC12 cell lysate, (6) Rat heart lysate.

Figure 1. Western blot analysis of anti-beta III Tubulin/TUBB3 antibody (BM3881). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SH-SY5Y whole cell lysates,
Lane 2: human SiHa whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-beta III Tubulin/TUBB3 antigen affinity purified monoclonal antibody (BM3881) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for beta III Tubulin/TUBB3 at approximately 50 kDa. The expected band size for beta III Tubulin/TUBB3 is at 50 kDa.

Figure 2. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human testicular seminoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human testicular seminoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 5. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 6. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 7. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human tonsil tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 8. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 9. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human urothelial carcinoma tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 10. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 11. IHC analysis of beta III Tubulin/TUBB3 using anti-beta III Tubulin/TUBB3 antibody (BM3881) .
beta III Tubulin/TUBB3 was detected in a paraffin-embedded section of human non-small cell lung cancer tissue. The tissue section was incubated with rabbit anti-beta III Tubulin/TUBB3 Antibody (BM3881) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Western blot analysis of beta Tubulin expression in (1) Jurkat cell lysate, (2) Human kidney lysate, (3) 3T3 cell lysate, (4) Mouse brain lysate, (5) C6 cell lysate, (6) Rat heart lysate.

Figure 3. Flow cytometry analysis of SiHa cell (1:100) DyLight 488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight 488. Unlabelled sample (Red line).

Figure 1. Western blot analysis of anti- TUBB antibody (M01857-3). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysates,Lane 2: human HELA whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human HL-60 whole cell lysates,Lane 5: human Raji whole cell lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse brain tissue lysates.Use rabbit anti- TUBB 1:1000, probed with a goat anti- mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001). A specific band was detected for TUBB at approximately 55KD. The expected band size for TUBB is at 55KD.

Figure 2. ICC analysis using anti- TUBB antibody (M01857-3). was detected in immersion fixed A431 cell. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog # BA1126) and counterstained with DAPI (blue).

Figure 1. Western blot analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human SiHa whole cell lysates,
Lane 2: human 293T whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: monkey COS-7 whole cell lysates,
Lane 5: chicken heart tissue lysates,
Lane 6: rat brain tissue lysates,
Lane 7: rat PC-12 whole cell lysates,
Lane 8: mouse brain tissue lysates,
Lane 9: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-Beta Tubulin/TUBB antigen affinity purified polyclonal antibody (A01857-1) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for Beta Tubulin/TUBB at approximately 50 kDa. The expected band size for Beta Tubulin/TUBB is at 50 kDa.

Figure 2. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
Beta Tubulin/TUBB was detected in a paraffin-embedded section of human liver cancer tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IHC analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1) .
Beta Tubulin/TUBB was detected in a paraffin-embedded section of human testicular germ cell tumors tissue. The tissue section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 4. IF analysis of Beta Tubulin/TUBB using anti-Beta Tubulin/TUBB antibody (A01857-1).
Beta Tubulin/TUBB was detected in an immunocytochemical section of U2OS cells. The section was incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Figure 5. Flow Cytometry analysis of SiHa cells using anti-Beta Tubulin/TUBB antibody (A01857-1).
Overlay histogram showing SiHa cells stained with A01857-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Beta Tubulin/TUBB Antibody (A01857-1) at 1:100 dilution for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG at 1:100 dilution used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Figure 1. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 tissue lysates,Lane 2: monkey COS-7 whole cell lysates,Lane 3: human PC-3 whole cell lysates,Lane 4: human Hela whole cell lysates,Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.

Figure 2. Western blot analysis of anti- Tubulin beta antibody (M05613-4).The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat liver whole cell lysates,Lane 3: rat PC-12 whole cell lysates,Lane 4: mouse brain whole cell lysates,Lane 5: mouse NIH3T3, whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.Use mouse anti- Tubulin beta 1:1000, probed with a goat anti-mouse IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for Tubulin beta at approximately 55KD. The expected band size for Tubulin beta is at 55KD.

Figure 5. Flow cytometry analysis of U937 cell(1:100) DyLight488 conjugated goat anti-mouse IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was mouse IgG DyLight488. Unlabelled sample (Red line).

Figure 3. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

Figure 4. ICC analysis of anti- Tubulin beta antibody (M05613-4).was detected in immunocytochemical section of U2OS cells. Cells were stained using the Dylight488-conjugated Anti-mouse IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).

Figure 1. Western blot analysis of anti-β-tubulin antibody (A05397-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human HEL whole cell lysates,
Lane 4: human HepG2 whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-β-tubulin antigen affinity purified polyclonal antibody (A05397-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for β-tubulin at approximately 55 kDa. The expected band size for β-tubulin is at 50 kDa.

Figure 2. IHC analysis of β-tubulin using anti-β-tubulin antibody (A05397-1).
β-tubulin was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

Figure 3. IF analysis of β-tubulin using anti-β-tubulin antibody (A05397-1).
β-tubulin was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

Figure 4. Flow Cytometry analysis of SiHa cells using anti-β-tubulin antibody (A05397-1).
Overlay histogram showing SiHa cells stained with A05397-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-β-tubulin Antibody (A05397-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.