Figure 1. Western blot analysis of anti- KCNQ1 antibody (A15277-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- KCNQ1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for KCNQ1 at approximately 75KD. The expected band size for KCNQ1 is at 75KD.
Figure 2. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 3. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 4. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 5. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(7) 
Figure 6. ICC analysis using anti-KCNQ1 antibody (A00310-1) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
all(7) 
Figure 7. Flow cytometry analysis of U937 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(7) | Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- KCNQ1 antibody (A15277-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human THP-1 whole cell lysates,
Lane 2: rat stomach tissue lysates,
Lane 3: rat lung tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse stomach tissue lysates,
Lane 6: mouse lung tissue lysates,
Lane 7: mouse NIH/3T3 whole cell lysates.
Use rabbit anti- KCNQ1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for KCNQ1 at approximately 75KD. The expected band size for KCNQ1 is at 75KD.
Figure 2. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human liver cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human placenta tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 5. IHC analysis using anti-KCNQ1 antibody (A00310-1). detected in paraffin-embedded section of human breast cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 6. ICC analysis using anti-KCNQ1 antibody (A00310-1) was detected in immersion fixed HELA cell line . Cells were stained using the Dylight488-conjugated Anti-rabbit IgG Secondary Antibody (green)(Catalog#BA1127) and counterstained with DAPI (blue).
Figure 7. Flow cytometry analysis of U937 cell (1:100) DyLight 488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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