Anti-4EBP1/EIF4EBP1 Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB IHC FCM

A00968-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- EIF4EBP1 Antibody (A00968-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: HEL whole cell lysates,
Lane 2: PC-3 whole cell lysates,
Lane 3: HEK293 whole cell lysates,
Lane 4: rat stomach tissue lysates,
Lane 5: rat pancreas tissue lysates,
Lane 6: mouse pancreas tissue lysates.
Use rabbit anti- EIF4EBP1 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for EIF4EBP1 at approximately 17KD. The expected band size for EIF4EBP1 is at 13KD.
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Figure 2. IHC analysis using anti- EIF4EBP1 Antibody (A00968-1). detected in paraffin-embedded section of human lung cancer tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. Flow cytometry analysis of A431 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
all(3)

产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-4EBP1/EIF4EBP1 Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

4E BP1; 4EBP1; BP 1; eIF4E binding protein 1; EIF4EBP1; PHAS I

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, IHC, FCM

基因名称

EIF4EBP1

抗体来源

Rabbit

抗体亚型

IgG

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human EIF4EBP1, which shares 75% and 81.2% amino acid (aa) sequence identity with mouse and rat EIF4EBP1, respectively.

计算分子量

13 kDa

实际分子量

17 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

Eukaryotic translation initiation factor 4E-binding protein 1 (also known as 4E-BP1) is a protein that in humans is encoded by the EIF4EBP1 gene. This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.

Uniprot ID

Q13541

RRID

AB_3081303

文献引用格式

4EBP1/EIF4EBP1 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A00968-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

eukaryotic translation initiation factor 4E binding protein 1

蛋白名全称

Eukaryotic translation initiation factor 4E-binding protein 1

实验方案>

实验步骤

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