Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human PANC-1 whole cell lysates,Lane 4: human SK-OV-3 whole cell lysates,Lane 5: human COLO-320 whole cell lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse testis tissue lysates. anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMC6L1 at approximately 126KDThe expected band size for SMC6L1 is at 126KD.
all(6)
Figure 2. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 3. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 4. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
all(6) 
Figure 5. Flow Cytometry analysis of A431 cells using anti- SMC6L1 antibody (A01554-1). Overlay histogram showing A431 cells stained with A01554-1 (Blue line). anti- SMC6L1 Antibody A01554-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
all(6) 
Figure 6. IF analysis of SMC6L1 using anti- SMC6L1 antibody (A01554-1).
SMC6L1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- SMC6L1 Antibody (A01554-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human PANC-1 whole cell lysates,Lane 4: human SK-OV-3 whole cell lysates,Lane 5: human COLO-320 whole cell lysates,Lane 6: rat testis tissue lysates,Lane 7: mouse testis tissue lysates. anti-SMC6L1 antigen affinity purified polyclonal antibody (Catalog # A01554-1)probed with a goat anti-rabbit IgG-HRP secondary antibody The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) . A specific band was detected for SMC6L1 at approximately 126KDThe expected band size for SMC6L1 is at 126KD.
Figure 2. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of rat testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of human testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 4. IHC analysis of SMC6L1 using anti-SMC6L1 antibody (A01554-1). SMC6L1 was detected in paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ugμg/ml rabbit anti-SMC6L1 Antibody (A01554-1) . Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 5. Flow Cytometry analysis of A431 cells using anti- SMC6L1 antibody (A01554-1). Overlay histogram showing A431 cells stained with A01554-1 (Blue line). anti- SMC6L1 Antibody A01554-1, 1:100) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody . Isotype control antibody (Green line) was rabbit IgG (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 6. IF analysis of SMC6L1 using anti- SMC6L1 antibody (A01554-1).
SMC6L1 was detected in immunocytochemical section of A431 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) rabbit anti- SMC6L1 Antibody (A01554-1) . DyLight488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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