Figure 1. Western blot analysis of anti- NPC2 antibody (A01582-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates.
Use rabbit anti- NPC2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NPC2 at approximately 19KD. The expected band size for NPC2 is at 16KD.
Figure 2. IHC analysis of anti-NPC2 antibody (A01582-3). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(5) 
Figure 3. IHC analysis of anti-NPC2 antibody (A01582-3). detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
all(5) 
Figure 4. Flow cytometry analysis of HepG2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
all(5) 
Figure 5. IF analysis of NPC2 using anti-NPC2 antibody (A01582-3).
NPC2 was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunocytochemistry/Immunofluorescence (ICC/IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| Enzyme linked immunosorbent assay (ELISA): | 1:100-1000 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of anti- NPC2 antibody (A01582-3). The sample well of each lane was loaded with 30ug of sample under reducing conditions.
Lane 1: human U20S whole cell lysates.
Use rabbit anti- NPC2 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog#EK1002). A specific band was detected for NPC2 at approximately 19KD. The expected band size for NPC2 is at 16KD.
Figure 2. IHC analysis of anti-NPC2 antibody (A01582-3). detected in paraffin-embedded section of human tonsil tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 3. IHC analysis of anti-NPC2 antibody (A01582-3). detected in paraffin-embedded section of mouse spleen tissue. Biotinylated goat anti-rabbit IgG was used as secondary antibody . The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Figure 4. Flow cytometry analysis of HepG2 cell(1:100) DyLight488 conjugated goat anti-rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight488. Unlabelled sample (Red line).
Figure 5. IF analysis of NPC2 using anti-NPC2 antibody (A01582-3).
NPC2 was detected in an immunocytochemical section of A431 cells. DyLight®488 Conjugated Goat Anti-Rabbit IgG (Green) (Catalog # BA1127) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
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