产品名称
Anti-2B4/CD244 Antibody
规格/价格
50μl/1180 100μl/1960 150μl/2780
指标别称
2B4; CD244; h2B4; NAIL; NKR2B4; SLAMF4
免疫原
E.coli-derived rat 2B4/Cd244 recombinant protein (Position: Q20-Q372).
成分
500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.
纯化方式
Immunogen affinity purified.
保存条件
12 months from date of receipt,-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
背景资料
CD244(Cluster of Differentiation 244) is a human protein encoded by the CD244 gene. It is also known as Natural Killer Cell Receptor 2B4. Tangye et al.(1999) mapped the 2B4 gene to 1q22. Suzuki et al.(2008) identified a functional single-nucleotide polymorphism(SNP) in the CD244 gene that contributes to rheumatoid arthritis susceptibility. Functional analysis by Boles et al.(1999) demonstrated that engagement of 2B4 with specific antibody activates NK cytolytic activity. Using recombinant human NK cell-activating ligand 2B4 fused to domains 3 and 4 of rodent Cd4 and flow cytometric analysis, Brown et al.(1998) demonstrated that CD48 binds to 2B4. Watzl et al.(2000) showed that antibody-mediated cross-linking of 2B4 leads to its rapid tyrosine phosphorylation, which is necessary for 2B4-mediated killer cell activity.
文献引用格式
2B4/CD244 Antibody (Boster Biological Technology, Wuhan, China. Catalog#A02527-2)
应用释义
WB-蛋白质免疫印迹法; IHC-免疫组织化学法; ICC/IF-免疫细胞荧光; ICC-免疫细胞化学; IHC-F-冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;
推荐配套的二抗和检测试剂
Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). *Blocking peptide 可以联系我们购买。
推荐稀释比
Western blot (WB): | 1:500-2000 |
ELISA: | 1:100-1000 |