Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

Rabbit polyclonal antibody

说明书

筛选器： All WB FCM ELISA

A04148-1

50μl ￥1180 100μl ￥1960 150μl ￥2780
货期：现货

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Figure 1. Western blot analysis of anti- YWHAG antibody (A04148-1). The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: Mouse brain tissue lysates.Use rabbit anti- YWHAG 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for YWHAG at approximately 28KD. The expected band size for YWHAG is at 28KD.
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Figure 2. Flow cytometry analysis of A549 cell (1:100) DyLight 488 conjugated goat anti- rabbit IgG(blue) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG DyLight 488. Unlabelled sample (Red line).
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产品简介 实验方案 引用文献 相关产品

产品简介>

产品名称

Anti-14-3-3 GAMMA/YWHAG-Specific Antibody

规格/价格

50μl/1180 100μl/1960 150μl/2780

指标别称

14 3 3 protein gamma; 14 3 3GAMMA; 14-3-3; 14-3-3 GAMMA; KCIP 1; YWHAG

产品类型

Polyclonal

检验物种

human, mouse, rat

应用范围

WB, FCM, ELISA

基因名称

YWHAG

抗体来源

Rabbit

抗体亚型

IgG

免疫原

E.coli-derived human 14-3-3 gamma/YWHAG recombinant protein (Position: Y107-A138).

计算分子量

28 kDa

实际分子量

28 kDa

成分

500 ug/ml antibody with PBS, 0.02% NaN3, 1 mg/ml BSA and 50% glycerol.

纯化方式

Immunogen affinity purified.

浓度

500 ug/ml

产品形态

Liquid

保存条件

12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.

背景资料

This gene product belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 100% identical to the rat ortholog. It is induced by growth factors in human vascular smooth muscle cells, and is also highly expressed in skeletal and heart muscles, suggesting an important role for this protein in muscle tissue. It has been shown to interact with RAF1 and protein kinase C, proteins involved in various signal transduction pathways.

Uniprot ID

P61981

文献引用格式

14-3-3 GAMMA/YWHAG-Specific Antibody (Boster Biological Technology, Wuhan, China. Catalog#A04148-1)

应用释义

WB-蛋白质免疫印迹法; IHC- 免疫组织化学法; ICC/IF-免疫细胞荧光; ICC－免疫细胞化学; IHC-F- 冰冻切片免疫组化; FCM-流式细胞术; ELISA-酶联免疫吸附测定; IP-免疫沉淀法; IF-免疫组织荧光法; ChIP-染色质免疫沉淀法;

基因名全称

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein gamma

蛋白名全称

14-3-3 protein gamma

实验方案>

实验步骤

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