Anti-Beta Tubulin/TUBB1 Antibody

筛选器： All WB IHC ICC/IF FCM ELISA

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  Figure 1. Western blot analysis of anti-β-tubulin antibody (A05397-1). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human K562 whole cell lysates,
  Lane 3: human HEL whole cell lysates,
  Lane 4: human HepG2 whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: mouse brain tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-β-tubulin antigen affinity purified polyclonal antibody (A05397-1) and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for β-tubulin at approximately 55 kDa. The expected band size for β-tubulin is at 50 kDa.

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  Figure 2. IHC analysis of β-tubulin using anti-β-tubulin antibody (A05397-1).
  β-tubulin was detected in a paraffin-embedded section of human rectal cancer tissue. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.

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  Figure 3. IF analysis of β-tubulin using anti-β-tubulin antibody (A05397-1).
  β-tubulin was detected in an immunocytochemical section of U2OS cells. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).

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  Figure 4. Flow Cytometry analysis of SiHa cells using anti-β-tubulin antibody (A05397-1).
  Overlay histogram showing SiHa cells stained with A05397-1 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-β-tubulin Antibody (A05397-1, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.