Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (BA3638). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (BA3638) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
Figure 2. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of CD68 using anti-CD68 antibody (BA3638).
CD68 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. IF analysis of CD68 using anti-CD68 antibody (BA3638).
CD68 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody.
Figure 9. Flow Cytometry analysis of RAW264.7 cells using anti-CD68 antibody (BA3638).
Overlay histogram showing RAW264.7 cells stained with BA3638 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD68 Antibody (BA3638, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
| Western blot (WB): | 1:500-2000 |
| Immunohistochemistry (IHC): | 1:50-400 |
| Immunofluorescence (IF): | 1:50-400 |
| Flow Cytometry (Fixed): | 1:50-200 |
| (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. | |
Figure 1. Western blot analysis of CD68 using anti-CD68 antibody (BA3638). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse spleen tissue lysates,
Lane 3: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-CD68 antigen affinity purified polyclonal antibody (BA3638) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for CD68 at approximately 90-100 kDa. The expected band size for CD68 is at 37 kDa.
Figure 2. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 3. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 4. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of mouse spleen tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 5. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 6. IHC analysis of CD68 using anti-CD68 antibody (BA3638) .
CD68 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:200 and developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB (Catalog # AR1022) as the chromogen.
Figure 7. IF analysis of CD68 using anti-CD68 antibody (BA3638).
CD68 was detected in a paraffin-embedded section of rat liver tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody. The section was counterstained with DAPI (Catalog # AR1176) (Blue).
Figure 8. IF analysis of CD68 using anti-CD68 antibody (BA3638).
CD68 was detected in a paraffin-embedded section of rat lung tissue. The tissue section was incubated with rabbit anti-CD68 Antibody (BA3638) at a dilution of 1:100. Cy3-conjugated Anti-rabbit IgG Secondary Antibody (red)(Catalog#BA1032) was used as secondary antibody.
Figure 9. Flow Cytometry analysis of RAW264.7 cells using anti-CD68 antibody (BA3638).
Overlay histogram showing RAW264.7 cells stained with BA3638 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD68 Antibody (BA3638, 1:100). DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 1:100) was used as secondary antibody. Isotype control antibody (Green line) was rabbit IgG (Catalog # BA1045) (1:100) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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