Anti-14-3-3 alpha/beta Antibody

筛选器： All WB IHC ICC/IF IP FCM

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  Figure 1. Western blot analysis of anti-14-3-3 alpha/beta antibody (BM4752). The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
  Lane 1: human Hela whole cell lysates,
  Lane 2: human Caco-2 whole cell lysates,
  Lane 3: human SiHa whole cell lysates,
  Lane 4: human Hacat whole cell lysates,
  Lane 5: rat brain tissue lysates,
  Lane 6: rat lung tissue lysates,
  Lane 7: mouse brain tissue lysates,
  Lane 8: mouse lung tissue lysates.
  After electrophoresis, proteins were transferred to a membrane. Then the membrane was incubated with rabbit anti-14-3-3 alpha/beta antigen affinity purified monoclonal antibody (BM4752) at a dilution of 1:1000 and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054). The signal is developed using ECL Plus Western Blotting Substrate (Catalog # AR1197). A specific band was detected for 14-3-3 alpha/beta at approximately 28 kDa. The expected band size for 14-3-3 alpha/beta is at 28 kDa.