Human Activin A ELISA Kit

96T/2480

Activin beta A chain; EDF; FRP; INHBA; Inhibin Beta A; Inhibin beta A chain; Inhibin β-A; inhibin; beta A

CHO, G311-S426

There is no detectable cross-reactivity with other relevant proteins.

<12 pg/ml

15.6 pg/ml - 1000 pg/ml

INHBA

A4D1W7

Human Activin A ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0301)

Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.

25-30分钟

Catalog number	Description	Quantity
EK0301-CAP	96-well plate precoated with anti- human Activin A antibody	1
EK0301-ST	Lyophilized recombinant human Activin A standard	10ng/tube×2
EK0301-DA	Biotinylated anti- human Activin A antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	34	199	467	33	205	482
Standard deviation	1.63	15.72	29.88	2.54	14.76	35.66
CV(%)	4.8	7.9	6.4	7.7	7.2	7.4

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.037	0.073	0.085	0.147	0.289	0.537	1.066	2.200

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Saliva

3.5h