Bovine BMP5 ELISA Kit

96T/2480

BMP 5; BMP5; BMP-5; bone morphogenetic protein 5

Expression system for standard: NSO; Immunogen sequence: A317-H454

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

156 pg/ml - 10000 pg/ml

BMP5

Bone morphogenetic protein 5

P22003

Bovine BMP5 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0310-BV)

Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFbeta superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on Bovine chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.

25-30分钟

Catalog number	Description	Quantity
EK0310-BV-CAP	Anti-Human BMP5 Pre-coated 96-well strip microplate	1
EK0310-BV-ST	Human BMP5 Standard	10ng/tube×2
EK0310-BV-DA	Human BMP5 Biotinylated antibody (100x)	100µl
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	100µl
AR1106-1	Sample Diluent	30ml
AR1106-2	Antibody Diluent	12ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	12ml
AR1104	Color Developing Reagent (TMB)	10ml
AR1105	Stop solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(ng/ml)	1.23	3.46	6.92	1.38	3.57	6.85
Standard deviation	0.059	0.180	0.388	0.073	0.203	0.425
CV(%)	4.8	5.2	5.6	5.3	5.7	6.2

Concentration(pg/ml)	0	156	312	625	1250	2500	5000	10000
O.D.	0.056	0.099	0.116	0.201	0.348	0.634	1.135	1.946

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum and Bone Tissue

3.5h