Rat BMP2 ELISA Kit

96T/2480

BMP-2; BMP 2; BMP 2A; BMP2; BMP2A; bone morphogenetic protein 2; Bone morphogenetic protein 2A

Expression system for standard: CHO; Immunogen sequence: Q283-R396

There is no detectable cross-reactivity with other relevant proteins.

<2pg/ml

31.2pg/ml-2000pg/ml

BMP2

bone morphogenetic protein 2

P49001

Rat BMP2 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0312)

Bone morphogenetic protein-2 (BMP-2, BMP 2A) belongs to the transforming growth factor beta (TGF-beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice. BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase (MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis. Bone morphogenic proteins (BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion. The standard product used in this kit is recombinant rat BMP-2, constituting dimer by two chains of 114 amino acids with the molecular mass of 26KDa.

Catalog number	Description	Quantity
EK0312-CAP	96-well plate precoated with anti- rat BMP-2 antibody	1
EK0312-ST	Lyophilized recombinant rat BMP-2 standard	10ng/tube×2
EK0312-DA	Biotinylated anti- rat BMP-2 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex (ABC)	100μl(dilution 1:100)
AR1106-1	Sample diluent buffer	30 ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	96	245	806	105	234	777
Standard deviation	6.72	11.27	49.16	9.24	14.97	52.83
CV(%)	7%	4.6%	6.1%	8.8%	6.4%	6.8%

Concentration(pg/ml)	0	31.2	62.5	125	250	500	1000	2000
O.D.	0.017	0.069	0.116	0.215	0.454	0.992	2.008	2.506

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

rat

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum