Human IL10 ELISA Kit

96T/2280

CSIF, IL 10, IL10, IL-10, IL10A, interleukin 10, TGIF

E.coli, S19-N178

There is no detectable cross-reactivity with other relevant proteins.

<0.5 pg/ml

7.8 pg/ml - 500 pg/ml (cellculturesupernates) 3.4 pg/ml - 250 pg/ml (humanserumplasma)

IL10

interleukin 10

P22301

Human IL10 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0416)

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation.1 The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1. The standard product used in this kit is recombinant human IL-10, consisting of 160 amino acids with the molecular mass of 18.6KDa.

20-25分钟

Catalog number	Description	Quantity
EK0416-CAP	96-well plate precoated with anti- human IL-10 antibody	1
EK0416-ST	Lyophilized recombinant human IL-10 standard	10ng/tubex2
EK0416-DA	Biotinylated anti- human IL-10 antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	16	53	113	15	54	122
Standard deviation	1.02	2.12	7.34	1.24	3.02	9.88
CV(%)	6.4	4	6.5	8.3	5.6	8.1

Concentration(pg/ml)	0	7.8	15.6	31.3	62.5	125	250	500
O.D.	0.023	0.080	0.126	0.226	0.424	0.749	1.388	2.493

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum and Plasma (heparin, EDTA, citrate)

3.5h