Human ACE ELISA Kit

96T/2480

ACE; ACE1; Angiotensin converting enzyme; CD143; DCP; DCP1; Dipeptidyl carboxypeptidase I; Kininase II; MVCD3

NSO, L30-L1261

There is no detectable cross-reactivity with other relevant proteins.

<5 pg/ml

0.78 ng/ml - 50 ng/ml

ACE

angiotensin I converting enzyme

P12821

Human ACE ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0557)

Angiotensin-converting enzyme(ACE) is a zinc-containing dipeptidyl carboxypeptidase widely distributed in mammalian tissues and is thought to play a critical role in blood pressure regulation. The predicted protein is identical, from residue 37 to its C terminus, to the second half or C-terminal domain of the endothelial ACE sequence. The protein sequence inferred consists of a 732-residue preprotein including a 31-residue signal peptide. The mature polypeptide has a molecular weight of 80,073. Although ACE has been studied primarily in the context of its role in blood pressure regulation, this widely distributed enzyme has many other physiological functions. The ACE gene encodes two isozymes. The somatic isozyme is expressed in many tissues, including vascular endothelial cells, renal epithelial cells, and testicular Leydig cells, whereas the testicular or germinal angiotensin-converting enzyme is expressed only in sperm. The standard product used in this kit is recombinant human ACE, consisting of 30-1261 amino acids with the molecular mass of 120KDa.

25-30分钟

Catalog number	Description	Quantity
EK0557-CAP	96-well plate precoated with anti- human ACE antibody	1
EK0557-ST	Lyophilized recombinant human ACE standard	50ng/tubex2
EK0557-DA	Biotinylated anti- human ACE antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	1278	6839	24157	1358	6350	25528
Standard deviation	62.62	465.05	1087.06	88.27	482.6	1225.34
CV(%)	4.9	6.8	4.5	6.5	7.6	4.8

Concentration(ng/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.043	0.137	0.223	0.399	0.685	1.116	1.577	2.300

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Serum, Plasma (heparin) and Saliva

3.5h