Human Adiponectin/ADIPOQ ELISA Kit

96T/2480

ACDC; ACRP30; adiponectin; ADIPOQ; ADIPQTL1; ADPN; APM 1; APM1; GBP28; Gelatin binding protein

NSO, E19-N244

There is no detectable cross-reactivity with other relevant proteins.

<60 pg/ml

1.56 ng/ml - 100 ng/ml

ADIPOQ

adiponectin, C1Q and collagen domain containing

Q15848

Human Adiponectin/ADIPOQ ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0595)

Adiponectin(ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226(19-244) amino acids with the molecular mass of 36KDa after glycosylation.

20-25分钟

Catalog number	Description	Quantity
EK0595-CAP	96-well plate precoated with anti- human Adiponectin antibody	1
EK0595-ST0	Lyophilized recombinant human Adiponectin standard	100ng/tubex2
EK0595-DA	Biotinylated anti- human Adiponectin antibody	100ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	3726	12058	49927	3646	11478	49590
Standard deviation	182.57	940.52	2346.56	236.99	1033.02	2578.68
CV(%)	4.9	7.8	4.7	6.5	9	5.2

Concentration(ng/ml)	0	1.56	3.12	6.25	12.5	25	50	100
O.D.	0.003	0.074	0.149	0.369	0.509	0.862	1.510	2.059

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

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Cell Culture Supernatants, Serum, Plasma (heparin, EDTA) and Urine

3.5h