Human ADAM8 ELISA Kit

96T/2480

ADAM metallopeptidase domain 8; ADAM8; ADAM8 protein; CD156; MS2

NSO, M1-P497

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

62.5 pg/ml - 4000 pg/ml

ADAM8

ADAM metallopeptidase domain 8

P78325

Human ADAM8 ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0650)

A Disintegrin and metalloproteinase domain-containing protein 8 is an enzyme that in humans is encoded by the ADAM8 gene. ADAM8 is localized to chromosome 10q26.3. This gene encodes a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of the ADAM family, such as ADAM8, are cell surface proteases involved in remodeling of extracellular matrix, cell migration, and processing of membrane-bound signaling molecules. They are characterized by disintegrin and metalloprotease domains that confer adhesive properties and proteolytic activities, respectively. And the protein encoded by this gene may be involved in cell adhesion duringneurodegeneration.

15-20分钟

Catalog number	Description	Quantity
EK0650-CAP	96-well plate precoated with anti- human ADAM8antibody	1
EK0650-ST	Lyophilized recombinant human ADAM8 standard	10ng/tube×2
EK0650-DA	Biotinylated anti- human ADAM8 antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	143	493	1805	150	511	1879
Standard deviation	10.72	27.6	97.47	11.25	33.72	127.77
CV(%)	7.5	5.6	5.4	7.5	6.6	6.8

Concentration(pg/ml)	0	62.5	125	250	500	1000	2000	4000
O.D.	0.006	0.060	0.129	0.256	0.442	0.829	1.384	1.979

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants and Serum

3.5h