Human AFP ELISA Kit

96T/2480

AFP; Alpha 1 fetoprotein; Alpha fetoglobulin; alpha fetoprotein; FETA; HPAFP

NSO, R19-V609

There is no detectable cross-reactivity with other relevant proteins.

<10 pg/ml

78 pg/ml - 5000 pg/ml

AFP

alpha fetoprotein

P02771

Human AFP ELISA Kit (Boster Biological Technology, Wuhan, China. Catalog#EK0651)

Alpha-fetoprotein (AFP, α-fetoprotein; also sometimes called alpha-1-fetoprotein, alpha-fetoglobulin, or alpha fetal protein) is a protein that in humans is encoded by the AFP gene. The AFP gene is located on the q arm of chromosome 4 (4q25). AFP is the most abundant plasma protein found in the human fetus. Plasma levels decrease rapidly after birth but begin decreasing prenatally starting at the end of the first trimester. Normal adult levels are usually achieved by the age of 8 to 12 months. The function of AFP in adult humans is unknown; however, in rodents it binds estradiol to prevent the transport of this hormone across the placenta to the fetus. The main function of this is to prevent the virilization of female fetuses. As human AFP does not bind estrogen, its function in humans is less clear.

15-20分钟

Catalog number	Description	Quantity
EK0651-CAP	96-well plate precoated with anti- human AFP antibody	1
EK0651-ST	Lyophilized recombinant human AFP standard	5ng/tube×2
EK0651-DA	Biotinylated anti- human AFP antibody	100μl(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	100ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	95	643	2190	98	654	1986
Standard deviation	4.56	47.58	100.74	5.58	56.24	129.09
CV(%)	4.8	7.4	4.6	5.7	8.6	6.5

Concentration(pg/ml)	0	78	156	312	625	1250	2500	5000
O.D.	0.003	0.137	0.248	0.486	0.777	1.260	1.704	1.925

Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

ELISA

human

酶联免疫双抗夹心法

Cell Culture Supernatants, Cell Lysates, Serum and Plasma (heparin, EDTA)

3.5h